Koehler J K
Am J Anat. 1977 Jun;149(2):135-51. doi: 10.1002/aja.1001490202.
The fine structure of spermatozoa of the Asian musk shrew, Suncus murinus, has been investigated using air-dried and critical-point-dried replicas in addition to freeze-fractured specimens. Asymmetry in the structure of the head, notable the acrosome, can be recognized in critical-point-dried replicas as a slight dorsoventral convexity not apparent in air-dried specimens. Freeze-etching reveals a dense accumulation of intramembranous particles just anterior to the posterior ring, but relatively few other characteristic membrane conformations associated with the head. Gelatin smears visualized with the light microscope show a vigorous lytic response, but preliminary efforts to remove the acrosome by physiological or enzymatic incubation have thus far not been successful.