Kinsey W H, Koehler J K
J Supramol Struct. 1976;5(2):185-98. doi: 10.1002/jss.400050207.
The plasma membrane of epididymal spermatozoa of the golden hamster (Mesocricetus auratus) exhibits morphological differences over various parts of the head and tail as detected by air-dried replicas and freeze-etching techniques. In an attempt to ascertain whether any topographical differences exist in the number or distribution of carbohydrate moieties associated with the cell surface, cells were labeled with Concanavalin A and marked with hemocyanin. It was found that while the plasma membrane over the acrosomal region differed from that of the postacrosomal region in membrane components revealed by freeze fracturing, there was no apparent difference in the distribution or density of Con A binding sites detectable by hemocyanin localization. The tail regions exhibited differences in both fracture face appearance and the distribution of detectable carbohydrate moieties. It was also found that binding sites for Concanavalin A exist on the inner and outer acrosomal membranes in addition to those on the plasma membrane.
通过空气干燥复制品和冷冻蚀刻技术检测发现，金黄地鼠（Mesocricetus auratus）附睾精子的质膜在头部和尾部的不同部位呈现出形态差异。为了确定与细胞表面相关的碳水化合物部分的数量或分布是否存在任何拓扑差异，细胞用伴刀豆球蛋白A进行标记并用血蓝蛋白进行标记。结果发现，虽然冷冻断裂显示顶体区域的质膜与顶体后区域的质膜在膜成分上有所不同，但通过血蓝蛋白定位检测到的伴刀豆球蛋白A结合位点的分布或密度没有明显差异。尾部区域在断裂面外观和可检测到的碳水化合物部分的分布上均表现出差异。还发现，除了质膜上的结合位点外，顶体内外膜上也存在伴刀豆球蛋白A的结合位点。
