Bucala R, Spiegel L A, Chesney J, Hogan M, Cerami A
Picower Institute for Medical Research, Manhasset, New York 11030, USA.
Mol Med. 1994 Nov;1(1):71-81.
The host response to tissue injury requires a complex interplay of diverse cellular, humoral, and connective tissue elements. Fibroblasts participate in this process by proliferating within injured sites and contributing to scar formation and the longterm remodeling of damaged tissue. Fibroblasts present in areas of tissue injury generally have been regarded to arise by recruitment from surrounding connective tissue; however this may not be the only source of these cells.
Long-term culture of adherent, human, and murine leukocyte subpopulations was combined with a variety of immunofluorescence and functional analyses to identify a blood-borne cell type with fibroblast-like properties.
We describe for the first time a population of circulating cells with fibroblast properties that specifically enter sites of tissue injury. This novel cell type, termed a "fibrocyte," was characterized by its distinctive phenotype (collagen+/vimentin+/CD34+), by its rapid entry from blood into subcutaneously implanted wound chambers, and by its presence in connective tissue scars.
Blood-borne fibrocytes contribute to scar formation and may play an important role both in normal wound repair and in pathological fibrotic responses.
机体对组织损伤的反应需要多种细胞、体液和结缔组织成分之间复杂的相互作用。成纤维细胞通过在损伤部位增殖并参与瘢痕形成和受损组织的长期重塑来参与这一过程。组织损伤部位的成纤维细胞通常被认为是由周围结缔组织募集而来；然而，这可能不是这些细胞的唯一来源。
将贴壁的人源和鼠源白细胞亚群进行长期培养，并结合多种免疫荧光和功能分析，以鉴定具有成纤维细胞样特性的血源性细胞类型。
我们首次描述了一群具有成纤维细胞特性的循环细胞，它们能特异性地进入组织损伤部位。这种新型细胞类型，称为“纤维细胞”，其特征在于独特的表型（胶原蛋白+/波形蛋白+/CD34+），能迅速从血液进入皮下植入的伤口腔室，并存在于结缔组织瘢痕中。
血源性纤维细胞有助于瘢痕形成，可能在正常伤口修复和病理性纤维化反应中都发挥重要作用。
