Golde D W, Hocking W G, Quan S G, Sparkes R S, Gale R P
Br J Haematol. 1980 Feb;44(2):183-7. doi: 10.1111/j.1365-2141.1980.tb01200.x.
We investigated the origin of bone marrow fibroblasts in three bone marrow transplant recipients with aplastic anaemia and leukaemia who received grafts from HLA-identical siblings of opposite sex. The patients were conditioned for transplantation with high doses of cytotoxic drugs and 300--1000 rads total body irradiation. After transplantation, bone marrow cells were cultured in T flasks for 3 weeks and the adherent cells were then trypsinized and passaged weekly. After several passages the cells had the typical morphology and growth pattern of fibroblasts. Metaphases from these cells were all of recipient sex type. In contrast, haematopoietic cells and lymphocytes obtained at the same time were of donor sex type. Our findings indicate that the human bone marrow fibroblast is not derived from a precursor common to haemotopoietic cells or lymphocytes. The bone marrow fibroblast appears to be a mesenchymal cell, unrelated to haematopoietic stem cells, which is capable of in vitro proliferation after as much as 1000 rads of total body irradiation.
我们研究了3例再生障碍性贫血和白血病患者骨髓成纤维细胞的来源，这些患者接受了来自异性 HLA 相同同胞的移植。患者接受高剂量细胞毒性药物和300 - 1000拉德全身照射预处理以进行移植。移植后，将骨髓细胞在T瓶中培养3周，然后将贴壁细胞用胰蛋白酶消化并每周传代。经过几次传代后，细胞具有成纤维细胞的典型形态和生长模式。这些细胞的中期染色体均为受体性别类型。相比之下，同时获得的造血细胞和淋巴细胞为供体性别类型。我们的研究结果表明，人类骨髓成纤维细胞并非源自造血细胞或淋巴细胞的共同前体。骨髓成纤维细胞似乎是一种间充质细胞，与造血干细胞无关，在高达1000拉德的全身照射后仍能在体外增殖。
