Kelly P J, Masanvi N, Cadman H F, Mahan S M, Beati L, Raoult D
Department of Clinical Veterinary Studies, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe.
Avian Dis. 1996 Apr-Jun;40(2):448-52.
Sera from 216 ostriches on nine farms around Zimbabwe were examined by indirect fluorescent antibody (IFA) testing for the presence of antibodies reactive with Cowdria ruminantium, Coxiella burnetii, and Rickettsia africae, a spotted fever group rickettsia. Although no reactive antibodies could be detected to C. ruminantium or C. burnetii, 51/216 (35%) sera reacted with R. africae. The seroprevalence in ostriches from the south of Zimbabwe was significantly higher than in birds from the north (P < 0.01). Immunoblots of four sera positive by IFA (> 1/160) showed antibodies reactive with antigens of R. africae that also were recognized by pooled sera from mice inoculated with the organism. No reactive antibodies could be detected in six sera negative by IFA.
对津巴布韦各地9个农场的216只鸵鸟的血清进行了间接荧光抗体（IFA）检测，以检测是否存在与反刍兽考德里氏体、伯氏考克斯氏体以及斑点热群立克次体非洲立克次体发生反应的抗体。尽管未检测到与反刍兽考德里氏体或伯氏考克斯氏体发生反应的抗体，但216份血清中有51份（35%）与非洲立克次体发生反应。津巴布韦南部鸵鸟的血清阳性率显著高于北部的鸟类（P < 0.01）。4份IFA检测呈阳性（> 1/160）的血清的免疫印迹显示，与非洲立克次体抗原发生反应的抗体也被接种该病原体的小鼠混合血清所识别。在6份IFA检测呈阴性的血清中未检测到反应性抗体。
