Finidori J P, Raoult D, Bornstein N, Fleurette J
Unité des Rickettsies, Centre National de Référence, CHU La Timone, Marseille, France.
Acta Virol. 1992 Oct;36(5):459-65.
Patients with Q fever and legionellosis may present identical clinical symptom. Differentiation of these diseases is made by serology, mainly the indirect immunofluorescence assay (IFA). Using IFA the authors tested 154 Q fever positive sera from 55 patients with acute Q fever and 28 patients with chronic Q fever for Legionella pneumophila antibodies and 57 sera from 57 patients with legionellosis for Coxiella burnetii antibodies. Of the 211 sera tested, four sera from different patients had antibodies to both C. burnetii and L. pneumophila. Using cross-adsorption studies and protein immunoblotting, no cross-reaction between C. burnetii and L. pneumophila antibodies could be identified. The moderate antibody titers against L. pneumophila in two Q fever patients and vice versa for one legionellosis patient are consistent with the incidence of seroprevalence in healthy blood donors and were not due to cross-reactivity. One patient was identified with concurrent Q fever and legionellosis.
Q热和军团病患者可能表现出相同的临床症状。这些疾病的鉴别通过血清学检测，主要是间接免疫荧光测定法（IFA）。作者使用IFA检测了来自55例急性Q热患者和28例慢性Q热患者的154份Q热阳性血清中的嗜肺军团菌抗体，以及来自57例军团病患者的57份血清中的伯氏考克斯体抗体。在检测的211份血清中，来自不同患者的4份血清同时含有针对伯氏考克斯体和嗜肺军团菌的抗体。通过交叉吸附研究和蛋白质免疫印迹法，未发现伯氏考克斯体和嗜肺军团菌抗体之间存在交叉反应。两名Q热患者中针对嗜肺军团菌的抗体滴度中等，一名军团病患者针对伯氏考克斯体的抗体滴度中等，这与健康献血者中血清阳性率的发生率一致，并非交叉反应所致。一名患者被确诊同时患有Q热和军团病。
