Telischi M, Hoiberg R, Rao K R, Patel A R
Am J Clin Pathol. 1977 Aug;68(2):250-7. doi: 10.1093/ajcp/68.2.250.
A program of component therapy using largely frozen erythrocytes was initiated at Cook County Hospital in July 1973. Use of the three existing washing systems for routine preparation of frozen erythrocytes has shown that there are differences in the levels of free hemoglobin, hematocrit, and residual glycerol in the washed products. Adenosine triphosphate, 2,3-diphosphoglycerate, and extracellular potassium and sodium were found to be within acceptable limits. Some expired units were cultured and were found to be positive for Staphylococcus and Corynebacterium. The source of contamination has not been determined. Frozen blood, when available, has been given to all patients, regardless of age or clinical condition. The incidence of transfusion reactions has decreased from 0.57% prior to the inception of the component therapy program to 0.11% since that time. Two cases of possible posttransfusion hepatitis occurred in patients who had received non-frozen blood, and in three patients who received non-frozen erythrocytes and/or components as well as frozen blood. Although the goal of the program was the use of frozen erythrocytes exclusively, only 64% use was achieved, as sufficient quantities of blood for freezing were not available at all times.
1973年7月，库克县医院启动了一项主要使用冷冻红细胞的成分治疗计划。使用现有的三种洗涤系统对冷冻红细胞进行常规制备后发现，洗涤后的产品中游离血红蛋白、血细胞比容和残余甘油的水平存在差异。三磷酸腺苷、2,3 -二磷酸甘油酸以及细胞外钾和钠的含量均在可接受范围内。对一些过期单位进行培养后发现，它们对葡萄球菌和棒状杆菌呈阳性。污染来源尚未确定。只要有冷冻血液，就会给所有患者使用，无论其年龄或临床状况如何。输血反应的发生率已从成分治疗计划开始前的0.57%降至此后的0.11%。两例可能的输血后肝炎发生在接受非冷冻血液的患者中，另有三例发生在既接受非冷冻红细胞和/或成分又接受冷冻血液的患者中。尽管该计划的目标是仅使用冷冻红细胞，但由于并非随时都有足够数量的血液用于冷冻，实际使用率仅达到64%。
