Szymanski I O, Carrington E J
Transfusion. 1977 Sep-Oct;17(5):431-7. doi: 10.1046/j.1537-2995.1977.17578014579.x.
The characteristics of previously frozen red blood cells, prepared in a large-scale frozen blood program using the Red Cross method were evaluated. The use of the method as originally described resulted in approximately 91 per cent freeze-thaw-wash recovery of red blood cells. When the glycerolization step was modified by adding the partially glycerolized erythrocytes into 300 ml of 6.2M glycerol, freeze-thaw-wash recoveries were decreased. However, gradient addition of glycerol to the red blood cells without the use of stylet, resulted in acceptable in vitro recoveries. Thawing frozen units in waterbath, to which no antiseptic was added, could introduce bacteria into units of previously frozen red blood cells. Therefore, it seems advisable to use dry heat thawing procedures. Previously frozen red blood cells prepared in the large scale maintained normal levels of ATP and 2,3 DPG. Therapeutic transfusions had acceptable 24-hour survival in vivo.
对采用红十字会方法在大规模冷冻血液项目中制备的先前冷冻红细胞的特性进行了评估。按照最初描述的方法使用该方法，红细胞的冻融洗涤回收率约为91%。当通过将部分甘油化红细胞加入300 ml 6.2M甘油中来修改甘油化步骤时，冻融洗涤回收率降低。然而，在不使用探针的情况下向红细胞中梯度添加甘油，可获得可接受的体外回收率。在未添加防腐剂的水浴中解冻冷冻单位，可能会将细菌引入先前冷冻的红细胞单位。因此，使用干热解冻程序似乎是可取的。大规模制备的先前冷冻红细胞维持了正常水平的ATP和2,3-DPG。治疗性输血在体内24小时存活率可接受。
