Dubeski P L, Owens F N, Song W O, Coburn S P, Mahuren J D
Department of Animal Science, Oklahoma State University, Stillwater 74078-0425, USA.
J Anim Sci. 1996 Jun;74(6):1358-66. doi: 10.2527/1996.7461358x.
For nonruminants, stress and disease greatly increase requirements for vitamin B6, folic acid, pantothenic acid, and ascorbate. The effects of feed restriction, virus infection, and vitamin injections on plasma concentrations of B vitamins critical to the immune response were evaluated. Twelve beef steer calves, 6 to 8 mo of age, were fed below maintenance for 17 d and deprived of food for 3 d during a 20-d period after weaning. They then were inoculated intranasally with live attenuated bovine herpesvirus-1 (BHV-1). Six calves received saline injections and six received injections of a B vitamin mixture and ascorbate every 48 h for 14 d before and 14 d after inoculation. A mild respiratory infection developed in all calves 4 to 5 d after inoculation. In control calves, restricted intake and food deprivation decreased plasma vitamin B6 and pantothenate and increased vitamin B12 but did not affect folic acid and ascorbate concentrations. Vitamin injections increased plasma concentrations of vitamin B6, folic acid, vitamin B12, pantothenic acid, and ascorbate (P < .002). Plasma concentrations of vitamin B6, vitamin B12, pantothenic acid, and ascorbate, but not folic acid, were markedly reduced in all calves during the BHV-1 infection (P = .001). The vitamin B6, pantothenic acid, vitamin B12, and ascorbate status of stressed calves may affect their immune response to vaccination or infection.
对于非反刍动物而言，应激和疾病会大幅增加其对维生素B6、叶酸、泛酸和抗坏血酸的需求。研究评估了限饲、病毒感染及维生素注射对免疫反应至关重要的B族维生素血浆浓度的影响。选取12头6至8月龄的肉用小公牛犊，在断奶后的20天内，使其采食低于维持需要量的饲料达17天，并禁食3天。然后给它们经鼻接种减毒活牛疱疹病毒1型（BHV - 1）。6头犊牛注射生理盐水，另外6头在接种前14天和接种后14天每48小时注射一次B族维生素混合物及抗坏血酸。接种后4至5天，所有犊牛均出现轻度呼吸道感染。在对照犊牛中，采食量受限和禁食使血浆维生素B6和泛酸盐水平降低，维生素B12水平升高，但未影响叶酸和抗坏血酸浓度。维生素注射使血浆维生素B6、叶酸、维生素B12、泛酸和抗坏血酸浓度升高（P < 0.002）。在BHV - 1感染期间，所有犊牛血浆中的维生素B6、维生素B12、泛酸和抗坏血酸浓度均显著降低，但叶酸浓度未受影响（P = 0.001）。处于应激状态的犊牛的维生素B6、泛酸、维生素B12和抗坏血酸状况可能会影响它们对疫苗接种或感染的免疫反应。
