Altamirano-Lozano M, Alvarez-Barrera L, Basurto-Alcántara F, Valverde M, Rojas E
Laboratorio de Citogenética, Mutagénesis y Toxicología Reproductiva, UIBR Campo-II, FES-Zaragoza, México, D.F., México.
Teratog Carcinog Mutagen. 1996;16(1):7-17. doi: 10.1002/(SICI)1520-6866(1996)16:1<7::AID-TCM2>3.0.CO;2-M.
Effects of vanadium pentoxide (V2O5) treatment on reproductive function and testicular DNA in male mice were investigated. These functions were evaluated with fertility rate, implants, resorptions, sperm counts, motility, and morphology. The DNA damage in individual testis cells was analyzed by single-cell gel electrophoresis technique (COMET assay). V2O5 treatment resulted in a decrease in fertility rate, implantations, live fetuses, and fetal weight, and an increase in the number of resorptions/dam. Sperm count, motility, and morphology were impaired with the advancement of treatment. Vanadium treatment induced DNA damage depending on the dose in the testis cells that was expressed and detected as DNA migration in the COMET assay. The distribution of DNA migration among cells, a function of dose, revealed that the majority of cells of treated animals expressed more DNA damage than cells from control animals. It is concluded that vanadium pentoxide was a reprotoxic and genotoxic agent in mice.
研究了五氧化二钒（V2O5）处理对雄性小鼠生殖功能和睾丸DNA的影响。通过生育率、着床数、吸收数、精子计数、活力和形态来评估这些功能。采用单细胞凝胶电泳技术（彗星试验）分析单个睾丸细胞中的DNA损伤。V2O5处理导致生育率、着床数、活胎数和胎儿体重下降，吸收/损伤数增加。随着处理时间的延长，精子计数、活力和形态受到损害。钒处理在睾丸细胞中诱导DNA损伤，其程度取决于剂量，在彗星试验中表现为DNA迁移并被检测到。DNA迁移在细胞间的分布是剂量的函数，结果显示，与对照动物的细胞相比，经处理动物的大多数细胞表现出更多的DNA损伤。结论是五氧化二钒对小鼠具有生殖毒性和遗传毒性。
