Bertram E M, Wilkinson R G, Lee B A, Jilbert A R, Kotlarski I
Department of Microbiology and Immunology, University of Adelaide, Australia.
Vet Immunol Immunopathol. 1996 Jun 1;51(3-4):353-63. doi: 10.1016/0165-2427(95)05528-2.
Duck lymphocytes have not been classified into cells resembling B or T cells of mammals. Reagents used in the past to identify lymphocyte populations in other species have not been useful for this purpose and antibodies raised to duck immunoglobulin bind in high proportions to blood and organ lymphocytes of ducks as well as to their red blood cells. Here we report that a polyclonal rabbit antiserum reacting to the CD3 marker on human T cells has been used to identify duck T lymphocytes. These antibodies react with the intracytoplasmic portion of the human CD3 epsilon chain (amino acids 156-168), an epitope highly conserved between mammals. Immunohistochemical staining with this antiserum of sections of duck lymphoid organs and FACScan analysis of duck lymphoid cell suspensions identified a population of duck lymphocytes with a staining pattern similar to that seen for mammalian T cells. This anti-human CD3 immunoprecipitated a 23 kDa protein from a duck lymphoblast lysate: a size similar to the human CD3 epsilon chain. This is the first direct identification of duck T lymphocytes.
鸭淋巴细胞尚未被分类为类似于哺乳动物B细胞或T细胞的细胞。过去用于鉴定其他物种淋巴细胞群体的试剂在此并不适用，针对鸭免疫球蛋白产生的抗体与鸭的血液和器官淋巴细胞以及它们的红细胞有很高比例的结合。在此我们报告，一种与人类T细胞上的CD3标志物反应的兔多克隆抗血清已被用于鉴定鸭T淋巴细胞。这些抗体与人类CD3 ε链的胞质部分（氨基酸156 - 168）反应，该表位在哺乳动物之间高度保守。用这种抗血清对鸭淋巴器官切片进行免疫组织化学染色以及对鸭淋巴细胞悬液进行流式细胞仪分析，鉴定出一群鸭淋巴细胞，其染色模式与哺乳动物T细胞相似。这种抗人CD3抗体从鸭淋巴母细胞裂解物中免疫沉淀出一种23 kDa的蛋白质：其大小与人类CD3 ε链相似。这是对鸭T淋巴细胞的首次直接鉴定。
