White J F
Am J Physiol. 1977 Jun;232(6):E553-9. doi: 10.1152/ajpendo.1977.232.6.E553.
Double-barreled chloride-selective microelectrodes constructed by a new method have been used to determine intracellular chloride activity (aic1) in the absorptive cells of isolated, stripped proximal and distal segments of Amphiuma small intestine. Chloride was passively distributed across the mucosal membrane in fall and winter animals and aic1 is about 20 mM. In contrast summer animals actively accumulated chloride in both proximal and distal segments. Parallel measurements of intracellular chloride concentration (Cic1) reveal a very low apparent chloride activity coefficient (aic1/CiC1) indicating that all of the chloride is not free in the cytoplasm. The chloride activity in the bath immediately adjacent to the mucosa is higher than in the bulk solution.
采用一种新方法构建的双管氯化物选择性微电极，已用于测定蚓螈小肠分离的近端和远端剥除段吸收细胞内的氯化物活性（aic1）。在秋冬季节的动物中，氯化物被动分布于黏膜膜，aic1约为20 mM。相比之下，夏季动物在近端和远端段均主动积累氯化物。细胞内氯化物浓度（Cic1）的平行测量显示，表观氯化物活度系数（aic1/CiC1）非常低，这表明并非所有氯化物在细胞质中都是游离的。紧邻黏膜的浴液中的氯化物活性高于本体溶液中的氯化物活性。
