Swaak A J, van den Brink H G, Aarden L A
Department of Rheumatology, Dr. Daniel den Hoed Clinic, Rotterdam, The Netherlands.
Scand J Rheumatol. 1996;25(4):233-8. doi: 10.3109/03009749609069992.
Whole blood cell culture has great advantage over isolated peripheral blood mononuclear cell culture, because it needs only small amounts of blood and is fast to perform. The current report focuses on the measurement of IL-6 and TNF alpha produced by peripheral blood monocytes of patients with systemic lupus erythematosus (SLE) in the whole blood cell culture system. After an initial triggering with lipopolysaccharide (LPS), a specific stimulus for monocytes, a decreased production of IL-6 relative to the controls was observed. Dividing our SLE patients according to treatment with corticosteroids, overall the IL-6 production was decreased in the patients treated with corticosteroids. TNF alpha production was comparable with normals, with the exception of an increased spontaneous production and using LPS stimulus of 4 pg/ml. In the patients treated with corticosteroids a decreased TNF production was observed, in contrast to the non-treated patients in which an increased TNF production was found compared with the controls using LPS doses higher than 62 pg/ml. The impaired acute phase reaction (APR) that has been described in the literature, might be explained by our observation of a decreased production of mainly IL-6. However, also this study showed that treatment has a strong impact on ex vivo IL-6 and TNF production.
全血细胞培养相对于分离的外周血单个核细胞培养具有很大优势，因为它仅需要少量血液且操作快速。本报告重点关注在全血细胞培养系统中系统性红斑狼疮（SLE）患者外周血单核细胞产生的白细胞介素-6（IL-6）和肿瘤坏死因子α（TNFα）的测量。在用脂多糖（LPS）（一种针对单核细胞的特异性刺激物）进行初始刺激后，观察到相对于对照组，IL-6的产生减少。根据是否使用皮质类固醇治疗将我们的SLE患者进行分组，总体而言，接受皮质类固醇治疗的患者中IL-6的产生减少。TNFα的产生与正常人相当，但自发产生增加且使用4 pg/ml的LPS刺激除外。在接受皮质类固醇治疗的患者中观察到TNF产生减少，与之相反，在未接受治疗的患者中，使用高于62 pg/ml的LPS剂量时，与对照组相比发现TNF产生增加。文献中描述的急性期反应（APR）受损可能可以用我们观察到的主要是IL-6产生减少来解释。然而，本研究也表明治疗对体外IL-6和TNF的产生有很大影响。
