Fukui K, Hada T, Imanishi H, Iwasaki A, Hirano K, Higashino K
3rd Department of Internal Medicine, Hyogo College of Medicine, Japan.
Tumour Biol. 1996;17(5):320-4. doi: 10.1159/000217994.
Enzymic, immunological and lectin-binding properties of alkaline phosphatase (AP) produced in Caco-2 cells, a human colon carcinoma cell line, were investigated. The enzyme was very similar to fetal intestinal (meconium) AP in the enzymic and immunological properties, but different from fetal intestinal AP in lectin-binding properties; expression of the galactose moiety was altered in AP of Caco-2 cells, compared to that of fetal intestinal AP. These results indicate that AP of Caco-2 cells can be used in place of fetal intestinal AP when the enzymic properties of an AP of unknown origin are investigated, but cannot be used instead of fetal intestinal AP in the structural study of AP.
对人结肠癌细胞系Caco - 2细胞产生的碱性磷酸酶（AP）的酶学、免疫学和凝集素结合特性进行了研究。该酶在酶学和免疫学特性上与胎儿肠道（胎粪）AP非常相似，但在凝集素结合特性上与胎儿肠道AP不同；与胎儿肠道AP相比，Caco - 2细胞AP中半乳糖部分的表达发生了改变。这些结果表明，在研究来源不明的AP的酶学特性时，Caco - 2细胞AP可替代胎儿肠道AP使用，但在AP的结构研究中不能替代胎儿肠道AP。
