Kamiyama F, Yamazaki K, Kawamura K, Kohara M
Sekisui Chemical Co. Ltd., Osaka, Japan.
Biomed Chromatogr. 1996 May-Jun;10(3):105-10. doi: 10.1002/(SICI)1099-0801(199605)10:3<105::AID-BMC564>3.0.CO;2-I.
The solute retention mechanism of reversed-phase liquid chromatography is examined by using newly synthesized hydrophilic porous copolymer beads as a stationary phase. It is shown that when water is used as an eluent, the retention time for solutes such as alcohols, ketones and polyethylene glycols increases with temperature. Adsorption behaviour of polyethylene glycol onto the copolymer beads shows that the adsorption also increases with temperature. These observations are explained on the basis of the hypothesis that hydrophobic interaction plays a vital role in solute retention.
通过使用新合成的亲水性多孔共聚物微球作为固定相，研究了反相液相色谱的溶质保留机理。结果表明，当用水作为洗脱剂时，醇类、酮类和聚乙二醇等溶质的保留时间随温度升高而增加。聚乙二醇在共聚物微球上的吸附行为表明，吸附也随温度升高而增加。基于疏水相互作用在溶质保留中起关键作用这一假设，对这些观察结果进行了解释。
