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Simultaneous determination of vanillylmandelic, homovanillic and 5-hydroxyindoleacetic acids in human urine by thin layer chromatography.

作者信息

Alemany G, Gamundí A, Rosselló C, Rial R

机构信息

Dept. de Biologia i Ciencies de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.

出版信息

Biomed Chromatogr. 1996 May-Jun;10(3):144-5. doi: 10.1002/(SICI)1099-0801(199605)10:3<144::AID-BMC570>3.0.CO;2-S.

DOI:10.1002/(SICI)1099-0801(199605)10:3<144::AID-BMC570>3.0.CO;2-S
PMID:8792867
Abstract

A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with beta-(4-hydroxy-3-phenyl) acetic acid as the internal standard.

摘要

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