Nüesch-Inderbinen M T, Hächler H, Kayser F H
Department of Medical Microbiology, University of Zürich, Switzerland.
Eur J Clin Microbiol Infect Dis. 1996 May;15(5):398-402. doi: 10.1007/BF01690097.
A highly sensitive and specific method, termed PCR/NheI, for the detection of genes coding for SHV extended-spectrum beta-lactamases (ESBL) in clinical isolates is presented. It is based on polymerase chain reaction (PCR) amplification of the blaSHV genes, followed by restriction with NheI. Due to the glycine (positive 238) (SHV-non-ESBL)-->serine (position 238) (SHV-ESBL) mutation, only PCR fragments from the genes coding for SHV-ESBLs were cleaved. A commercially available test for ESBLs, the E test ESBL, identified 52% of our 29 clinical isolates carrying blaSHV-ESBL genes as ESBL producers.
本文介绍了一种高度灵敏且特异的方法，称为PCR/NheI，用于检测临床分离株中编码SHV超广谱β-内酰胺酶（ESBL）的基因。该方法基于blaSHV基因的聚合酶链反应（PCR）扩增，随后用NheI进行酶切。由于甘氨酸（第238位阳性）（SHV-非ESBL）到丝氨酸（第238位）（SHV-ESBL）的突变，只有编码SHV-ESBL的基因的PCR片段被切割。一种市售的ESBL检测方法，即Etest ESBL，将我们29株携带blaSHV-ESBL基因的临床分离株中的52%鉴定为ESBL产生菌。
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