Zbieranowski I, Le Riche J C, Jackson S M, Olivotto I
Division of Cytology, British Columbia Cancer Agency, Vancouver Clinic, Canada.
Anal Cell Pathol. 1992 Jan;4(1):13-24.
Thirteen patients with locally advanced or recurrent breast carcinoma were monitored during radiation therapy by multiple, sequential, fine-needle aspiration biopsies (FNAB) with flow cytometry. The material was analyzed for qualitative cytomorphological evidence of radiation effect and for DNA content and cell cycle alterations. DNA ploidy was not affected by the radiation therapy, although the aneuploid tumors showed an increased frequency of cell cycle alterations. The most common change seen was an increase in S-G2M (58%). Other changes included a decrease in the proliferative/growth fraction (17%) and no significant redistribution of cells (25%). There was a relationship between the initial proliferative activity of the tumors and the type of cell cycle change which occurred. Flow cytometric analysis was a better predictor of early clinical response than was cytomorphological assessment. Sequential FNAB with flow cytometry is an effective method of monitoring the response of breast cancer to radiation therapy.
通过多次连续细针穿刺活检（FNAB）及流式细胞术，对13例局部晚期或复发性乳腺癌患者在放射治疗期间进行了监测。对取材进行分析，以寻找放射效应的定性细胞形态学证据以及DNA含量和细胞周期改变情况。尽管非整倍体肿瘤显示细胞周期改变的频率增加，但DNA倍性不受放射治疗的影响。最常见的变化是S-G2M期增加（58%）。其他变化包括增殖/生长分数降低（17%）以及细胞无明显重新分布（25%）。肿瘤的初始增殖活性与发生的细胞周期变化类型之间存在关联。流式细胞术分析比细胞形态学评估能更好地预测早期临床反应。连续FNAB及流式细胞术是监测乳腺癌对放射治疗反应的有效方法。
