Serror P, Sonenshein A L
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Mol Microbiol. 1996 May;20(4):843-52. doi: 10.1111/j.1365-2958.1996.tb02522.x.
The product of the codY gene is required for nutritional repression of the Bacillus subtillis dipeptide permease operon (dpp), an operon expressed at early stationary phase in nutrient-rich medium. Though unrelated to any known DNA-binding protein, CodY was shown to bind specifically to the dpp promoter region. DNase I footprinting experiments revealed that the CodY-protected region encompasses the dpp transcription start site and overlaps with the region protected by another regulatory protein, AbrB. CodY and AbrB were found to compete, in vitro, for binding to the dpp promoter region. Binding of CodY was altered in mutants defective in nutritional regulation.
枯草芽孢杆菌二肽通透酶操纵子（dpp）在营养丰富的培养基中于稳定期早期表达，其营养阻遏需要codY基因的产物。尽管与任何已知的DNA结合蛋白无关，但已证明CodY能特异性结合dpp启动子区域。DNase I足迹实验表明，CodY保护的区域包含dpp转录起始位点，并与另一种调节蛋白AbrB保护的区域重叠。发现CodY和AbrB在体外竞争与dpp启动子区域的结合。在营养调节缺陷的突变体中，CodY的结合发生了改变。
