枯草芽孢杆菌遗传感受态的营养抑制需要CodY。
CodY is required for nutritional repression of Bacillus subtilis genetic competence.
作者信息
Serror P, Sonenshein A L
机构信息
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
出版信息
J Bacteriol. 1996 Oct;178(20):5910-5. doi: 10.1128/jb.178.20.5910-5915.1996.
Abstract
The acquisition of genetic competence by Bacillus subtilis is repressed when the growth medium contains Casamino Acids. This repression was shown to be exerted at the level of expression from the promoters of the competence-regulatory genes srfA and comK and was relieved in strains carrying a null mutation in the codY gene. DNase I footprinting experiments showed that purified CodY binds directly to the srfA and comK promoter regions.
摘要
当生长培养基中含有酪蛋白氨基酸时，枯草芽孢杆菌的遗传感受态的获得受到抑制。这种抑制作用在感受态调节基因srfA和comK启动子的表达水平上发挥作用，并且在携带codY基因无效突变的菌株中得到缓解。DNA酶I足迹实验表明，纯化的CodY直接与srfA和comK启动子区域结合。
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