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白鲸(Delphinapterus leucas)的微卫星

Microsatellites from the beluga whale Delphinapterus leucas.

作者信息

Buchanan F C, Friesen M K, Littlejohn R P, Clayton J W

机构信息

Freshwater Institute, Department of Fisheries and Oceans, Manitoba, Canada.

出版信息

Mol Ecol. 1996 Aug;5(4):571-5.

PMID:8794563
Abstract

Fifteen microsatellites were isolated from a beluga whale Delphinapterus leucas, genomic library. The microsatellites were amplified in 100 beluga obtained from two widely separated locations. An average of 8.6 alleles per locus were detected and the average heterozygosity was 0.65 with a range of 0.27-0.86. All microsatellites were polymorphic and 13 of the genotype distributions observed were in Hardy-Weinberg equilibrium. It was possible with these microsatellites to assign correctly individual whales to their stock-of-origin 98% of the time. Microsatellites were amplified in 15 other cetaceans with these beluga-derived primers.

摘要

从白鲸(白鲸属)的基因组文库中分离出15个微卫星。这些微卫星在从两个相距甚远的地点获取的100头白鲸中进行了扩增。每个位点平均检测到8.6个等位基因,平均杂合度为0.65,范围在0.27 - 0.86之间。所有微卫星都是多态性的,观察到的13种基因型分布处于哈迪 - 温伯格平衡。利用这些微卫星,98%的情况下能够正确地将个体鲸鱼归属于它们的起源种群。使用这些源自白鲸的引物在另外15种鲸类动物中扩增了微卫星。

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