Vral A, Thierens H, De Ridder L
Department for Anatomy, Embryology and Histology, University of Ghent, Belgium.
Int J Radiat Biol. 1996 Aug;70(2):171-6. doi: 10.1080/095530096145166.
Ataxia telangiectasia (AT) is an autosomal recessive disease characterized by a progressive neuronal degeneration, immunodeficiency, cancer proneness and an extreme sensitivity to ionizing radiation. In this work, micronucleus dose-response curves for lymphocytes of normal and AT individuals, exposed in G(zero) to low LET gamma-rays and high LET fast neutrons, are compared. After gamma-irradiation, the micronucleus yields for AT lymphocytes are strongly increased compared with controls. The micronucleus dose-response curve for AT cells shows a linear dependence instead of a linear-quadratic one which is found for normal cells. After neutron irradiation, the increase in micronucleus yield above controls is less pronounced than with gamma-rays and the micronucleus dose-response curves are linear, as expected. The high increase in micronucleus yield compared with controls after gamma-irradiation further suggests the application of the micronucleus assay as a diagnostic tool for ataxia telangiectasia.
共济失调毛细血管扩张症（AT）是一种常染色体隐性疾病，其特征为进行性神经元退化、免疫缺陷、易患癌症以及对电离辐射极度敏感。在这项研究中，对处于G（零）期的正常人和AT患者的淋巴细胞，分别给予低传能线密度的γ射线和高传能线密度的快中子照射后，比较了两者的微核剂量反应曲线。γ射线照射后，与对照组相比，AT淋巴细胞的微核产额显著增加。AT细胞的微核剂量反应曲线呈线性关系，而正常细胞的则呈线性二次关系。中子照射后，与γ射线照射相比，微核产额高于对照组的增幅不那么明显，且微核剂量反应曲线呈线性，这与预期相符。γ射线照射后微核产额相比对照组的大幅增加，进一步表明微核试验可作为共济失调毛细血管扩张症的一种诊断工具。
