通过多重体外扩增毒力相关基因对牛源紧密黏附性大肠杆菌进行分型
Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes.
作者信息
China B, Pirson V, Mainil J
机构信息
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liège, Belgium.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3462-5. doi: 10.1128/aem.62.9.3462-3465.1996.
Abstract
Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method.
摘要
紧密黏附性大肠杆菌是犊牛腹泻的一种新致病原。其主要毒力因子是由eaeA基因编码的紧密黏附素蛋白和由slt基因编码的志贺样毒素。由于这些基因的序列是已知的，我们选择了特异性引物来扩增每个毒力基因，以便开发一种基于毒力相关基因多重扩增的新鉴定试验。在30株受试菌株中，14株eaeA阳性，15株eaeA阳性且slt-I阳性，1株eaeA阳性且slt-I和slt-II均阳性，1株eaeA阳性且slt-II阳性。我们发现该方法快速、特异，且与杂交方法结果完全一致。
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