通过离子和非离子表面活性剂洗脱病毒。
Elution of viruses by ionic and nonionic surfactants.
作者信息
Fujito B T, Lytle C D
机构信息
Thomas Jefferson High School for Science and Technology, Alexandria, Virginia 22312, USA.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3470-3. doi: 10.1128/aem.62.9.3470-3473.1996.
Abstract
The ionic and nonionic surfactants sodium dodecyl sulfate and Triton X-100, respectively, eluted two viruses, phi X174 and PRD1, which were adsorbed to the ionic and nonionic binding membranes cationic polysulfone and nitrocellulose, respectively. Results indicated that complete elution was readily achieved only when combinations of surfactants and binding membranes were matched (i.e., ionic-ionic or nonionic-nonionic).
摘要
离子型表面活性剂十二烷基硫酸钠和非离子型表面活性剂曲拉通X-100分别洗脱了两种病毒，即φX174和PRD1，它们分别吸附在离子型结合膜阳离子聚砜和非离子型结合膜硝化纤维素上。结果表明，只有当表面活性剂和结合膜的组合相匹配时（即离子型-离子型或非离子型-非离子型），才能很容易实现完全洗脱。
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