一种从草绿色链球菌和金黄色葡萄球菌中分离克隆载体和内源质粒的简单方法。
A simple procedure for isolation of cloning vectors and endogenous plasmids from viridans group streptococci and Staphylococcus aureus.
作者信息
Vriesema A J, Zaat S A, Dankert J
机构信息
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
出版信息
Appl Environ Microbiol. 1996 Sep;62(9):3527-9. doi: 10.1128/aem.62.9.3527-3529.1996.
Abstract
Isolation of plasmid DNA from viridans group streptococci is difficult, and preparations are often heavily contaminated with chromosomal DNA. We developed a simple protocol to isolate pure plasmid DNA for use in different molecular techniques, including automated sequencing. The protocol is also applicable for plasmid isolation from Staphylococcus aureus. In addition, the protocol allows isolation of pure endogenous plasmids from streptococci and S. aureus.
摘要
从草绿色链球菌中分离质粒DNA很困难，所制备的质粒常常被染色体DNA严重污染。我们开发了一种简单的方法来分离纯质粒DNA，用于包括自动测序在内的不同分子技术。该方法也适用于从金黄色葡萄球菌中分离质粒。此外，该方法还能从链球菌和金黄色葡萄球菌中分离出纯的内源质粒。
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