不同方法保存厚皮马拉色菌（旧称厚皮糠秕孢子菌）菌株的比较。

Comparison of different methods of maintenance of Malassezia pachydermatis (s. Pityrosporum pachydermatis) strains.

作者信息

Dworecka-Kaszak B, Toka F N

机构信息

Warsaw Agricultural University, Faculty of Veterinary Medicine, Department of Microbiology, Poland.

出版信息

Acta Microbiol Pol. 1996;45(1):103-5.

PMID:8795259

Abstract

The maintenance of Malassezia pachydermatis isolated from canine otitis and dermatitis was compared on Sabouraud Dextrose Agar slants under liquid paraffin, an broth and in water, and by paper replica method, silica gel method, freeze-drying and deep freezing at-80 degrees C. Hydrolase production before and after storage was determined with API-ZYM Bio-Merieux kits. Deep freezing and freeze-drying were the best methods of maintaining M. pachydermatis. Hydrolase production was stable throughout the maintenance period.

摘要

比较了从犬中耳炎和皮炎中分离出的厚皮马拉色菌在液体石蜡覆盖下的沙氏葡萄糖琼脂斜面、肉汤和水中的保存情况，以及通过纸片复制法、硅胶法、冻干和在-80℃深度冷冻的保存情况。使用API-ZYM生物梅里埃试剂盒测定保存前后的水解酶产生情况。深度冷冻和冻干是保存厚皮马拉色菌的最佳方法。在整个保存期间水解酶产生情况稳定。