Sekizaki H, Itoh K, Toyota E, Tanizawa K
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Chem Pharm Bull (Tokyo). 1996 Aug;44(8):1577-9. doi: 10.1248/cpb.44.1577.
A facile synthetic method for p-guanidinophenyl esters derived from a variety of amino acids and peptides, including D-amino acids, is presented. The kinetic behavior of trypsin towards these synthetic esters, inverse substrates, was analyzed. The spatial requirement of the enzyme active site for catalytic efficiency is discussed based on the steric characteristics of the substrates.
本文提出了一种简便的合成方法，用于制备源自多种氨基酸和肽（包括D-氨基酸）的对胍基苯酯。分析了胰蛋白酶对这些合成酯（反向底物）的动力学行为。基于底物的空间特征，讨论了酶活性位点对催化效率的空间要求。
