Sekizaki H, Itoh K, Toyota E, Tanizawa K
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Chem Pharm Bull (Tokyo). 1996 Aug;44(8):1585-7. doi: 10.1248/cpb.44.1585.
Trypsin-catalyzed peptide synthesis has been studied by using p-guanidinophenyl esters of N alpha-(tert-butyloxycarbonyl)amino acid and peptide as acyl donor components. The reaction conditions were optimized for organic solvents, pH, and concentration of acceptor. The method was especially useful for the preparation of various peptides containing D-amino acids. The enzymatic hydrolysis of the resulting products was negligible.
利用Nα-（叔丁氧羰基）氨基酸和肽的对胍基苯酯作为酰基供体成分，对胰蛋白酶催化的肽合成进行了研究。针对有机溶剂、pH值和受体浓度对反应条件进行了优化。该方法对于制备各种含有D-氨基酸的肽特别有用。所得产物的酶促水解可忽略不计。
