恶臭假单胞菌S-1水杨酸盐羟化酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of salicylate hydroxylase from Pseudomonas putida S-1.
作者信息
Yabuuchi T, Suzuki K, Sato T, Ohnishi K, Itagaki E, Morimoto Y
机构信息
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima.
出版信息
J Biochem. 1996 May;119(5):829-831. doi: 10.1093/oxfordjournals.jbchem.a021317.
Apo-salicylate hydroxylase from Pseudomonas putida S-1 has been crystallized by the dialysis method, using ammonium sulfate as the precipitant. The crystals belong to hexagonal space group P6(2) or P6(4) with unit cell dimensions of a = b = 142.8 A and c = 63.8 A, and diffract X-rays at higher than 3.5 A resolution. A heavy-atom derivative has been prepared by soaking a crystal in an ammonium sulfate solution containing p-chloromercuriphenylsulfonate.
恶臭假单胞菌S-1的载脂蛋白水杨酸羟化酶已通过透析法结晶，使用硫酸铵作为沉淀剂。晶体属于六方空间群P6(2)或P6(4)，晶胞参数为a = b = 142.8 Å，c = 63.8 Å，并且能在高于3.5 Å分辨率下衍射X射线。通过将晶体浸泡在含有对氯汞基苯磺酸盐的硫酸铵溶液中制备了重原子衍生物。
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