来自恶臭假单胞菌生物型B的酮甾体异构酶的结晶及初步X射线晶体学研究。

Crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from Pseudomonas putida biotype B.

作者信息

Oh B H, Kim S W, Ryu S E, Kim S S, Yoon M K, Choi K Y

机构信息

Department of Life Science, Pohang University of Science and Technology, Korea.

出版信息

Proteins. 1996 Apr;24(4):514-5. doi: 10.1002/(SICI)1097-0134(199604)24:4<514::AID-PROT10>3.0.CO;2-O.

DOI:10.1002/(SICI)1097-0134(199604)24:4<514::AID-PROT10>3.0.CO;2-O

PMID:8859999

Abstract

The delta(5)-3-ketosteroid isomerase from Pseudomonas putida biotype B has been crystallized. The crystals belong to the space group P2(1)2(1)2(1) with unit cell dimensions of a = 36.48 angstrom, b = 74.30 angstrom, c = 96.02 angstrom, and contain one homodimer per asymmetric unit. Native diffraction data to 2.19 Angstrom resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replacement.

摘要

恶臭假单胞菌生物型B的δ(5)-3-酮类固醇异构酶已被结晶。晶体属于空间群P2(1)2(1)2(1)，晶胞参数为a = 36.48埃，b = 74.30埃，c = 96.02埃，每个不对称单元包含一个同型二聚体。在室温下从一块晶体获得了分辨率为2.19埃的天然衍射数据，表明这些晶体非常适合通过多同晶置换法进行结构测定。