Wagner E P, Smith B W, Winefordner J D
Department of Chemistry, University of Florida, Gainesville 32611, USA.
Anal Chem. 1996 Sep 15;68(18):3199-203. doi: 10.1021/ac9603587.
Laser-excited atomic fluorescence has been used to detect lead that was electrothermally atomized from whole blood in a graphite furnace. A 9 kHz repetition rate copper vapor laser pumped dye laser was used to excite the lead at 283.3 nm, and the resulting atomic fluorescence was detected at 405.8 nm. No matrix modification was used other than a 1:21 dilution of the whole blood with high-purity water. Using the atomic fluorescence peak area as the analytical measure and a background correction technique based upon a simultaneous measurement of the transmitted laser intensity, excellent agreement for NIST and CDC certified whole blood reference samples was obtained with aqueous standards. A limit of detection in blood of 10 fg/mL (100 ag absolute) was achieved.
激光激发原子荧光已被用于检测在石墨炉中从全血电热雾化产生的铅。使用一台重复频率为9千赫兹的铜蒸气激光泵浦染料激光器在283.3纳米处激发铅，并在405.8纳米处检测产生的原子荧光。除了用高纯水将全血按1:21稀释外，未使用任何基体改进剂。以原子荧光峰面积作为分析测量指标，并采用基于同时测量透射激光强度的背景校正技术，对于美国国家标准与技术研究院（NIST）和美国疾病控制与预防中心（CDC）认证的全血参考样品，与水溶液标准品获得了极佳的一致性。实现了血液中10飞克/毫升（绝对量为100阿托克）的检测限。
