抗黄曲霉毒素B1抗体的产生。
Production of antibody against aflatoxin B1.
作者信息
Chu F S, Ueno I
出版信息
Appl Environ Microbiol. 1977 May;33(5):1125-8. doi: 10.1128/aem.33.5.1125-1128.1977.
Abstract
Antibody against aflatoxin B1 was obtained after one multiple-site injection of bovine serum albumin-aflatoxin B1 conjugate into rabbits. The antibody has greatest binding efficiency for aflatoxin B1, less efficiency for B2, G1, and Q1, and least for aflatoxicol, G2, and M1. Sterigmatocystin, coumarin, and 4-hydroxycoumarin did not give a cross-reaction with the antibody. The sensitivity of the binding assay for detection of aflatoxin B1 is in the range of 0.2 to 2.0 ng per 0.5-ml sample. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody titer determination are described.
摘要
通过将牛血清白蛋白-黄曲霉毒素B1偶联物多次多点注射到兔子体内后获得了抗黄曲霉毒素B1抗体。该抗体对黄曲霉毒素B1的结合效率最高，对B2、G1和Q1的效率较低，对黄曲霉毒素醇、G2和M1的效率最低。柄曲霉素、香豆素和4-羟基香豆素与该抗体没有交叉反应。用于检测黄曲霉毒素B1的结合测定法的灵敏度在每0.5毫升样品0.2至2.0纳克的范围内。文中描述了偶联物制备、免疫血清生产以及抗体效价测定方法的详细步骤。
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