抗赭曲霉毒素A抗体的产生。
Production of antibody against ochratoxin A.
作者信息
Chu F S, Chang F C, Hinsdill R D
出版信息
Appl Environ Microbiol. 1976 Jun;31(6):831-5. doi: 10.1128/aem.31.6.831-835.1976.
Abstract
Antibody against ochratoxin A was obtained after repeated injection of different protein-ochratoxin A conjugates to rabbits. Among many protein-ochratoxin conjugates tested, bovine serum albumin-ochratoxin A was found to be the best antigen. The antibody is specific for ochratoxins A, C, and T, but is not specific for ochratoxins B, alpha, and other coumarin derivatives. The sensitivity for ochratoxin A detection using a binding assay is in the range of 0.5 to 10 ng/0.5-ml sample. Detailed methods for the preparation of protein-ochratoxin conjugates, preparation of immune serum, and methods for antibody titers are described.
摘要
通过向兔子反复注射不同的蛋白质-赭曲霉毒素A偶联物获得了抗赭曲霉毒素A抗体。在测试的许多蛋白质-赭曲霉毒素偶联物中，发现牛血清白蛋白-赭曲霉毒素A是最佳抗原。该抗体对赭曲霉毒素A、C和T具有特异性，但对赭曲霉毒素B、α及其他香豆素衍生物不具有特异性。使用结合试验检测赭曲霉毒素A的灵敏度在0.5至10 ng/0.5 ml样品范围内。文中描述了蛋白质-赭曲霉毒素偶联物的制备、免疫血清的制备以及抗体效价测定的详细方法。
相似文献
1
Production of antibody against ochratoxin A.
Appl Environ Microbiol. 1976 Jun;31(6):831-5. doi: 10.1128/aem.31.6.831-835.1976.
2
[Radioimmunologic determination of the mycotoxin ochratoxin A--a new diagnostic method in Czechoslovakia].
Vet Med (Praha). 1988 Feb;33(2):101-8.
3
4
A new monoclonal antibody detecting ochratoxin A at the picogram level.
Lett Appl Microbiol. 1996 Feb;22(2):103-5. doi: 10.1111/j.1472-765x.1996.tb01119.x.
5
Development of a sensitive enzyme-linked immunosorbent assay for the determination of ochratoxin A.
J Agric Food Chem. 2005 Aug 24;53(17):6947-53. doi: 10.1021/jf0513922.
6
Production of a monoclonal antibody against ochratoxin A and its application to immunochromatographic assay.
J Agric Food Chem. 2005 Nov 2;53(22):8447-51. doi: 10.1021/jf051681q.
7
Immunochemical method for ochratoxin A.
Methods Mol Biol. 2001;157:81-94. doi: 10.1385/1-59259-064-0:81.
8
Determination of ochratoxin A by polyclonal antibodies based sensitive time-resolved fluoroimmunoassay.
Arch Toxicol. 2006 Aug;80(8):481-5. doi: 10.1007/s00204-006-0112-2. Epub 2006 May 11.
9
[Determination of ochratoxin A by ELISA I. Study on the preparation of ochratoxin a antigen].
Wei Sheng Yan Jiu. 2002 Feb;31(1):53-4, backcover.
10
Ochratoxin A Detection on Antibody- Immobilized on BSA-Functionalized Gold Electrodes.
PLoS One. 2016 Jul 28;11(7):e0160021. doi: 10.1371/journal.pone.0160021. eCollection 2016.
引用本文的文献
1
Development of a Polyclonal Antibody for the Immunoanalysis of Ochratoxin A (OTA) by Employing a Specially Designed Synthetic OTA Derivative as the Immunizing Hapten.
Toxins (Basel). 2025 Aug 16;17(8):415. doi: 10.3390/toxins17080415.
2
Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.
Toxins (Basel). 2022 Jan 20;14(2):73. doi: 10.3390/toxins14020073.
3
Ochratoxin A: 50 Years of Research.
Toxins (Basel). 2016 Jul 4;8(7):191. doi: 10.3390/toxins8070191.
4
Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India.
Front Microbiol. 2015 May 26;6:511. doi: 10.3389/fmicb.2015.00511. eCollection 2015.
5
Immunochemical methods for ochratoxin A detection: a review.
Toxins (Basel). 2012 Apr;4(4):244-66. doi: 10.3390/toxins4040244. Epub 2012 Apr 13.
6
Enzyme-linked immunosorbent assay for detection of ochratoxin A.
Appl Environ Microbiol. 1981 Jun;41(6):1472-4. doi: 10.1128/aem.41.6.1472-1474.1981.
7
Production and characterization of aflatoxin B2a antiserum.
Appl Environ Microbiol. 1981 Feb;41(2):478-82. doi: 10.1128/aem.41.2.478-482.1981.
8
Production and characterization of antibody against aflatoxin Q1.
Appl Environ Microbiol. 1984 Mar;47(3):526-32. doi: 10.1128/aem.47.3.526-532.1984.
9
Radioimmunoassay of ochratoxin A in barley.
Appl Environ Microbiol. 1985 Aug;50(2):529-31. doi: 10.1128/aem.50.2.529-531.1985.
10
Detection of ochratoxin A in porcine kidneys by a monoclonal antibody-based radioimmunoassay.
Appl Environ Microbiol. 1987 Mar;53(3):514-8. doi: 10.1128/aem.53.3.514-518.1987.
本文引用的文献
1
Ochratoxin A: occurrence as natural contaminant of a corn sample.
Appl Microbiol. 1969 May;17(5):765-6. doi: 10.1128/am.17.5.765-766.1969.
2
Mycotoxins (ochratoxin A, citrinin, and sterigmatocystin) and toxigenic fungi in grains and other agricultural products.
J Agric Food Chem. 1972 Nov-Dec;20(6):1103-9. doi: 10.1021/jf60184a010.
3
A comparative study of the interaction of ochratoxins with bovine serum albumin.
Biochem Pharmacol. 1974 Mar 15;23(6):1105-13. doi: 10.1016/0006-2952(74)90011-2.
4
Studies on ochratoxins.
CRC Crit Rev Toxicol. 1974 Jan;2(4):499-524. doi: 10.3109/10408447309025706.
5
Synthesis of ochratoxins TA and TC, analogs of ochratoxins A and C.
Experientia. 1974 Feb 15;30(2):174-5. doi: 10.1007/BF01927717.
6
Ochratoxins.
Adv Appl Microbiol. 1973;16:97-109. doi: 10.1016/s0065-2164(08)70024-8.
7
Contamination of Swedish cereals with ochratoxin A.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Apr;82(2):301-2. doi: 10.1111/j.1699-0463.1974.tb02329.x.
Zotero 插件