抗赭曲霉毒素A抗体的产生。
Production of antibody against ochratoxin A.
作者信息
Chu F S, Chang F C, Hinsdill R D
出版信息
Appl Environ Microbiol. 1976 Jun;31(6):831-5. doi: 10.1128/aem.31.6.831-835.1976.
Abstract
Antibody against ochratoxin A was obtained after repeated injection of different protein-ochratoxin A conjugates to rabbits. Among many protein-ochratoxin conjugates tested, bovine serum albumin-ochratoxin A was found to be the best antigen. The antibody is specific for ochratoxins A, C, and T, but is not specific for ochratoxins B, alpha, and other coumarin derivatives. The sensitivity for ochratoxin A detection using a binding assay is in the range of 0.5 to 10 ng/0.5-ml sample. Detailed methods for the preparation of protein-ochratoxin conjugates, preparation of immune serum, and methods for antibody titers are described.
摘要
通过向兔子反复注射不同的蛋白质-赭曲霉毒素A偶联物获得了抗赭曲霉毒素A抗体。在测试的许多蛋白质-赭曲霉毒素偶联物中，发现牛血清白蛋白-赭曲霉毒素A是最佳抗原。该抗体对赭曲霉毒素A、C和T具有特异性，但对赭曲霉毒素B、α及其他香豆素衍生物不具有特异性。使用结合试验检测赭曲霉毒素A的灵敏度在0.5至10 ng/0.5 ml样品范围内。文中描述了蛋白质-赭曲霉毒素偶联物的制备、免疫血清的制备以及抗体效价测定的详细方法。
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本文引用的文献
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Synthesis of ochratoxins TA and TC, analogs of ochratoxins A and C.赭曲霉毒素TA和TC（赭曲霉毒素A和C的类似物）的合成
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