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磷酸化富含丝氨酸/精氨酸的蛋白质：200S大核核糖核蛋白颗粒组装中的限制因素。

Phosphorylated Ser/Arg-rich proteins: limiting factors in the assembly of 200S large nuclear ribonucleoprotein particles.

作者信息

Yitzhaki S, Miriami E, Sperling R, Sperling J

机构信息

Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8830-5. doi: 10.1073/pnas.93.17.8830.

DOI:10.1073/pnas.93.17.8830
PMID:8799112
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC38553/
Abstract

We have previously shown that specific nuclear pre-mRNA transcripts and their splicing products, as well as the general population of nuclear poly(A)+ RNA, are packaged in large nuclear ribonucleoprotein (InRNP) particles that sediment at the 200S region in sucrose gradients. The InRNP particles contain all uridine-rich small nuclear ribonucleoprotein complexes required for pre-mRNA splicing, as well as protein splicing factors. In this paper we show that all of the phosphorylated, mAb 104 detectable, Ser/Arg-rich essential splicing factors (SR proteins) in the nucleoplasm are integral components of the InRNP particles, whereas only part of the essential splicing factor U2AF65 (U2 snRNP auxiliary factor) and the polypyrimidine tract binding protein (PTB) are associated with these particles. This finding suggests a limiting role for SR proteins in the assembly of the InRNP particles. We further show that the structural integrity of InRNP particles is sensitive to variations in the phosphorylation levels of the SR proteins.

摘要

我们之前已经表明，特定的核前体mRNA转录本及其剪接产物，以及核内聚腺苷酸加尾RNA的总体，都被包装在大的核糖核蛋白（InRNP）颗粒中，这些颗粒在蔗糖梯度中沉降于200S区域。InRNP颗粒包含前体mRNA剪接所需的所有富含尿苷的小核糖核蛋白复合物，以及蛋白质剪接因子。在本文中，我们表明核质中所有磷酸化的、可被单克隆抗体104检测到的、富含丝氨酸/精氨酸的必需剪接因子（SR蛋白）都是InRNP颗粒的组成成分，而只有部分必需剪接因子U2AF65（U2小核糖核蛋白辅助因子）和多嘧啶序列结合蛋白（PTB）与这些颗粒相关。这一发现表明SR蛋白在InRNP颗粒组装中起限制作用。我们进一步表明，InRNP颗粒的结构完整性对SR蛋白磷酸化水平的变化敏感。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/eede37e25e9b/pnas01521-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/679fc1473a51/pnas01521-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/efacca9a244f/pnas01521-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/ea608df1c115/pnas01521-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/5a2933037b00/pnas01521-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/a809d2cbe15f/pnas01521-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/eede37e25e9b/pnas01521-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/679fc1473a51/pnas01521-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/efacca9a244f/pnas01521-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/ea608df1c115/pnas01521-0045-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/5a2933037b00/pnas01521-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/a809d2cbe15f/pnas01521-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3457/38553/eede37e25e9b/pnas01521-0046-c.jpg

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