Malendowicz L K, Neri G, Nussdorfer G G, Nowak M, Filipiak K, Warchol J B
Department of Histology and Embryology, School of Medicine, Poznan, Poland.
Endocr Res. 1996 May;22(2):175-84. doi: 10.1080/07435809609030506.
Substance P (SP) did not change basal corticosterone (B) secretion of dispersed zona fasciculata-reticularis cells of the rat adrenal cortex. Conversely, spantide II (SPA), an antagonist of SP receptors, at a concentration 10(-7)/10(-6) M markedly raised it, and the effect was annulled by equimolar concentrations of SP. Both SP and SPA (10(-6) M) increased cytosolic free calcium concentration in our cell preparations; however, the response to SP was immediate, while that to SPA showed a lag-period of 4-5 min. SP concentration-dependently (from 10(-8) M to 10(-5) M) partially inhibited maximally ACTH (10(-8) M)-induced stimulation of B secretion of dispersed cells, and unexpectedly a similar effect was observed after SPA exposure. In light of these findings, the conclusion is drawn that SP, under basal conditions, does not exert a direct modulatory action of B secretion of rat adrenocortical cells. However, the possibility remains to be explored that SP may play a role in quenching, via a receptor-independent mechanism, the exceedingly high glucocorticoid responses to ACTH of rat adrenocortical cells.
P物质（SP）不会改变大鼠肾上腺皮质束状带-网状带分散细胞的基础皮质酮（B）分泌。相反，SP受体拮抗剂Ⅱ型速激肽（SPA）在浓度为10⁻⁷/10⁻⁶ M时可显著提高基础皮质酮分泌，且等摩尔浓度的SP可消除该作用。SP和SPA（10⁻⁶ M）均可增加我们细胞制剂中的胞质游离钙浓度；然而，对SP的反应是即时的，而对SPA的反应则有4-5分钟的延迟期。SP浓度依赖性地（从10⁻⁸ M至10⁻⁵ M）部分抑制最大促肾上腺皮质激素（10⁻⁸ M）诱导的分散细胞B分泌刺激，并且在暴露于SPA后意外地观察到类似的效果。根据这些发现，得出的结论是，在基础条件下，SP对大鼠肾上腺皮质细胞的B分泌不发挥直接调节作用。然而，SP可能通过一种不依赖受体的机制在抑制大鼠肾上腺皮质细胞对促肾上腺皮质激素的极高糖皮质激素反应中发挥作用，这一可能性仍有待探索。
