Courtheyn D, Moermans R, Schilt R, Boenke A
Rijksontledingslaboratorium (ROL), Gentbrugge, Belgium.
Food Addit Contam. 1996 Jul;13(5):493-509. doi: 10.1080/02652039609374436.
New projects of the European Commission, Measurement and Testing Programme (BCR) were set up in order to develop a modular sample preparation system for the determination of beta-agonists and animal feeds. Three phases are included: an extraction study, a clean-up study and finally a Second Intercomparison. This paper describes the extraction study in which four laboratories were involved. A total of 33 extraction conditions were tested regarding their yield on clenbuterol and salbutamol, their compatibility towards several clean-up and chromatographic end-methods and the influence of undesired coextractives. The conditions differed with respect to five factors: with or without organic solvent, temperature, pH, agitation and centrifugation. Their influence was examined via a ruggedness-test approach. A unique set-up allowed the combination of individual results in a complete factorial design. The addition of an organic solvent was found to be the most important factor. Interactions between factors were also studied. The best combinations of factors regarding the extraction are given. Finally limits for applicability and influence of organic solvents, pH and temperature were evaluated in a fifth laboratory towards enzyme immunoassay as detection method.
欧盟委员会测量与测试计划（BCR）设立了新项目，旨在开发一种用于测定β-激动剂和动物饲料的模块化样品制备系统。该项目包括三个阶段：萃取研究、净化研究以及最后的第二次比对。本文描述了有四个实验室参与的萃取研究。针对共33种萃取条件，测试了它们对克伦特罗和沙丁胺醇的提取率、对几种净化及色谱最终方法的兼容性以及不期望的共萃取物的影响。这些条件在五个因素方面存在差异：有无有机溶剂、温度、pH值、搅拌和离心。通过耐用性测试方法研究了它们的影响。一种独特的设置允许在完全析因设计中组合各个结果。发现添加有机溶剂是最重要的因素。还研究了各因素之间的相互作用。给出了萃取方面最佳的因素组合。最后，在第五个实验室中评估了有机溶剂、pH值和温度对作为检测方法的酶免疫测定的适用性限制和影响。
