Schilt R, Hooijerink H, Courtheyn D, Boenke A
State Institute for Quality Control of Agricultural Products (RIKILT-DLO), Wageningen, The Netherlands.
Food Addit Contam. 1996 Jul;13(5):477-92. doi: 10.1080/02652039609374435.
The European Commission, Measurement and Testing Programme (BCR) has initiated a project to improve the methodology for analysis of beta-agonists in animal feeding stuffs. An intercomparison of methods for clenbuterol in animal feed is described. The study involved 13 European laboratories which analysed a blank feed and three feed samples with three different levels of clenbuterol contamination. The participants used a variety of extraction (organic or aqueous solvents), clean-up (liquid-liquid, silica and C-18 solid phase and immuno-affinity chromatography) and end-point detection (HPLC, GC-MS and TLC) steps. The purpose of this study was to identify and to quantify clenbuterol. The coefficient of variation from all the results for the low level (25 micrograms/kg) was 39%, for the intermediate level (100 micrograms/kg) 52% and for the high level (1000 micrograms/kg) 35%. The study showed that the initial extraction, the modular clean-up step and their compatibility to the HPLC and the GC-MS determination step were critical steps.
欧盟委员会测量与测试计划（BCR）启动了一个项目，以改进动物饲料中β-激动剂的分析方法。本文描述了动物饲料中克伦特罗分析方法的比对情况。该研究涉及13个欧洲实验室，这些实验室分析了一份空白饲料和三份含有三种不同克伦特罗污染水平的饲料样本。参与者采用了多种提取方法（有机溶剂或水性溶剂）、净化方法（液-液萃取、硅胶和C-18固相萃取以及免疫亲和色谱法）和终点检测方法（高效液相色谱法、气相色谱-质谱联用法和薄层色谱法）。本研究的目的是鉴定和定量克伦特罗。低水平（25微克/千克）所有结果的变异系数为39%，中等水平（100微克/千克）为52%，高水平（1000微克/千克）为35%。研究表明，初始提取、模块化净化步骤及其与高效液相色谱法和气相色谱-质谱联用法测定步骤的兼容性是关键步骤。
