Regulation of growth and DNA synthesis in mouse lymphoma L5178Y cells under conditions of nutrient depletion.

The effect of cultivation of L5178Y lymphoblasts to high density and in two kinds of nutrient-depleted medium on their growth was observed. It was found that in cell population grown to stationary phase the mitotic index and labeled thymidine incorporation dropped about 3 times in comparison with exponential control. Upon dilution with fresh medium the cells resume traverse the life cycle in partially synchronized manner. Cultivation of the cells in medium with 0-5% serum induced very rapid loss of their viability. Cultivation of the cells in medium without asparagine and with dialyzed serum caused significant slowing of their growth-rate and upon dilution with complete medium the cells entered the exponential rate of division in an asynchronous manner.