Delgado-Viscogliosi P, Brugerolle G, Viscogliosi E
Laboratoire de Biologie Comparée des Protistes, Centre National de la Recherche Scientifique, Université Blaise Pascal de Clermont-Ferrand II, Aubière, France.
Cell Motil Cytoskeleton. 1996;33(4):288-97. doi: 10.1002/(SICI)1097-0169(1996)33:4<288::AID-CM5>3.0.CO;2-5.
Using several specific monoclonal antibodies, we investigated the occurrence and distribution of different post-translationally modified tubulin during interphase and division of the primitive flagellated protist Trichomonas vaginalis. Immunoblotting and immunofluorescence experiments revealed that interphasic microtubular structures of T. vaginalis contained acetylated and glutamylated but non-tyrosinated and non-glycylated [Brugerolle and Adoutte, 1988: Bio Systems 21: 255-268] tubulin. Immunofluorescence studies performed on dividing cells showed that the extranuclear mitotic spindle (or paradesmosis) was acetylated and glutamylated, which contrast with the ephemeral nature of this structure. Newly formed short axostyles also contained acetylated and glutamylated tubulin suggesting that both post-translational modifications might take place very early after assembly of microtubular structures. Our results indicate that acetylation and glutamylation of tubulin appeared early in the history of eukaryotes and could reflect the occurrence of post-translational modifications of tubulin in the primitive eukaryotic cells. These cells probably had a highly ordered cross-linked microtubular cytoskeleton in which microtubules showed a low level of subunit exchange dynamics.
我们使用几种特异性单克隆抗体，研究了原始鞭毛原生生物阴道毛滴虫在间期和分裂过程中不同翻译后修饰微管蛋白的发生情况和分布。免疫印迹和免疫荧光实验表明，阴道毛滴虫的间期微管结构含有乙酰化和谷氨酰胺化的微管蛋白，但不含酪氨酸化和糖基化的微管蛋白[Brugerolle和Adoutte，1988：生物系统21：255 - 268]。对分裂细胞进行的免疫荧光研究表明，核外有丝分裂纺锤体（或平行纺锤体）是乙酰化和谷氨酰胺化的，这与该结构的短暂性质形成对比。新形成的短轴柱也含有乙酰化和谷氨酰胺化的微管蛋白，这表明这两种翻译后修饰可能在微管结构组装后很早就发生了。我们的结果表明，微管蛋白的乙酰化和谷氨酰胺化在真核生物历史早期就出现了，可能反映了原始真核细胞中微管蛋白翻译后修饰的发生情况。这些细胞可能具有高度有序的交联微管细胞骨架，其中微管显示出低水平的亚基交换动态。
