López-Medrano R, Ovejero M C, Calera J A, Puente P, Leal F
Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica, Universidad de Salamanca/C.S.I.C., Spain.
Eur J Clin Microbiol Infect Dis. 1996 Feb;15(2):146-52. doi: 10.1007/BF01591488.
At present there are no accepted criteria to assess the usefulness of Western blot assays for the serodiagnosis of aspergilloma. An Aspergillus fumigatus cytosolic fraction complex (CFC) composed of four proteins (p90, p60, p40, and p37) has been identified. The usefulness of Western blotting with CFC antigens for the serodiagnosis of aspergilloma was evaluated in 25 patients with well-established diagnoses and in 94 controls. The most consistently reactive antigen was p90 (92% of patients with aspergilloma), followed by p40 (76%) and the entire CFC taken together (76%). With these data, interpretive criteria for positive and negative immunoblots were established, with p90 indicated as a helpful marker of aspergilloma.
目前，尚无公认的标准来评估蛋白质印迹法在曲菌球血清学诊断中的有用性。已经鉴定出一种由四种蛋白质（p90、p60、p40和p37）组成的烟曲霉胞质组分复合物（CFC）。在25例确诊患者和94例对照中评估了使用CFC抗原进行蛋白质印迹法对曲菌球血清学诊断的有用性。反应最一致的抗原是p90（92%的曲菌球患者），其次是p40（76%）和整个CFC（76%）。根据这些数据，建立了免疫印迹阳性和阴性的解释标准，p90被认为是曲菌球的一个有用标志物。
