López-Medrano R, Ovejero M C, Calera J A, Puente P, Leal F
Departamento de Microbiología y Genética, Universidad de Salamanca/Consejo Superior de Investigaciones Cientificas, Spain.
Microbiology (Reading). 1995 Oct;141 ( Pt 10):2699-704. doi: 10.1099/13500872-141-10-2699.
Cytosolic fractions of mycelial extracts from Aspergillus nidulans, A. flavus, and three different isolates of A. fumigatus, grown to stationary phase in Czapek-Dox-AOAC medium, were tested by immunoblotting for the presence of antigens reactive to 80 serum samples from aspergilloma patients. Fifty control serum samples were used to determine the specificity of the reactions. In the A. fumigatus cytosolic fraction a group of four main antigenic bands (p90, p60, p40 and p37) was consistently recognized (in total or partial form) by 90% of the serum samples from the aspergilloma patients. This group of antigens was designated as the 'cytosolic fraction complex' (CFC). As confirmed by two-dimensional electrophoresis followed by immunoblotting with aspergilloma serum samples, each of the four antigenic bands is formed of several isoforms of acidic glycopeptides with slightly different pls. All the isoforms are at least N-glycosylated, as demonstrated by endoglycosidase H removal of a considerable amount of sugar residues. The relationship of these antigens with certain other A. fumigatus antigens previously reported in the literature, and their potential use in the immunodiagnosis of aspergilloma, are discussed.
将构巢曲霉、黄曲霉和三种不同烟曲霉分离株的菌丝提取物的胞质组分在查氏-杜氏-美国官方分析化学家协会（Czapek-Dox-AOAC）培养基中培养至稳定期，通过免疫印迹法检测其是否存在与80份曲霉菌瘤患者血清样本发生反应的抗原。使用50份对照血清样本确定反应的特异性。在烟曲霉胞质组分中，一组四条主要抗原带（p90、p60、p40和p37）始终被90%的曲霉菌瘤患者血清样本识别（全部或部分形式）。这组抗原被指定为“胞质组分复合物”（CFC）。通过二维电泳，然后用曲霉菌瘤血清样本进行免疫印迹法确认，四条抗原带中的每一条均由几种等电点略有不同的酸性糖肽同工型组成。如通过内切糖苷酶H去除大量糖残基所证明的，所有同工型至少进行了N-糖基化。讨论了这些抗原与文献中先前报道的某些其他烟曲霉抗原的关系，以及它们在曲霉菌瘤免疫诊断中的潜在用途。
