通过聚合酶链反应（PCR）检测组织培养物和蚊虫样本中的加利福尼亚血清群布尼亚病毒。
Detection of California serogroup Bunyaviruses in tissue culture and mosquito pools by PCR.
作者信息
Campbell W P, Huang C
机构信息
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509, USA.
出版信息
J Virol Methods. 1996 Apr 5;57(2):175-9. doi: 10.1016/0166-0934(95)01982-0.
A reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed for rapid detection of 14 California serogroup viruses using universal primer pairs. These primers are specific for the small RNA (S RNA) and middle RNA (M RNA) segments. The method has been employed to detect Jamestown Canyon (JC) virus in naturally infected mosquitoes. With this technique, it is possible to detect virus in an amount of material equivalent to as little as 0.03 mosquito.
开发了一种逆转录-聚合酶链反应（RT-PCR）方案，用于使用通用引物对快速检测14种加利福尼亚血清群病毒。这些引物对小RNA（S RNA）和中RNA（M RNA）片段具有特异性。该方法已用于检测自然感染蚊子中的詹姆斯敦峡谷（JC）病毒。使用这种技术，可以在相当于低至0.03只蚊子的材料量中检测到病毒。
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