Kuno G, Mitchell C J, Chang G J, Smith G C
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522-2087, USA.
J Clin Microbiol. 1996 May;34(5):1184-8. doi: 10.1128/jcm.34.5.1184-1188.1996.
Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. In this study, we evaluated eight pairs of primers for reactivity with 44 viruses of the genus Bunyavirus, using a reverse transcriptase PCR technique. With a pair of serogroup-specific primers we designed, all viruses of the serogroups tested could be detected. Further, virus-specific primer pairs were identified for California encephalitis virus, Jamestown Canyon virus, La Crosse virus, and snowshoe hare virus for use in North America. Using this technique, we could detect one La Crosse virus-infected mosquito in a pool of 100 mosquitoes with undetectable plaque titers.
布尼亚姆韦拉病毒群和加利福尼亚病毒群的许多布尼亚病毒都是对医学具有重要意义的人类病原体。利用分子生物学工具（如聚合酶链反应（PCR））开发一种有效的病毒检测技术，不仅能改善临床诊断，还能加强对野外蚊媒的病毒学监测。在本研究中，我们使用逆转录PCR技术评估了八对引物与44种布尼亚病毒属病毒的反应性。使用我们设计的一对血清群特异性引物，可以检测出所有测试血清群的病毒。此外，还确定了用于检测北美加利福尼亚脑炎病毒、詹姆斯敦峡谷病毒、拉克罗斯病毒和雪兔病毒的病毒特异性引物对。利用该技术，我们能够在100只蚊的混合样本中检测到一只感染拉克罗斯病毒的蚊子，而此时蚀斑滴定效价无法检测到。
