成年软骨细胞悬浮培养合成软骨特异性蛋白聚糖。
Synthesis of cartilage-specific proteoglycan by suspension cultures of adult chondrocytes.
作者信息
Wiebkin O W, Muir H
出版信息
Biochem J. 1977 Apr 15;164(1):269-72. doi: 10.1042/bj1640269.
Abstract
Chondrocytes isolated from larynges of adult pigs were cultured as cell suspensions for at least 4 days before use. During continuous-labelling experiments in nutrient medium for 18h with 35SO42- and [3H]glucosamine as precursors, some macromolecular polyanionic material was synthesized which behaved on gel chromatography as proteoglycan. Gel chromatography on Sepharose 2B showed that a proportion of the proteoglycans in the medium appeared to be aggregated, and was dissociated in 4M-guanidinium chloride. Moreover the dissociated proteoglycan interacted with hyaluronic acid. Newly synthesized proteoglycan was larger than the average total cetylpyridinium chloride-precipitable material assayed as uronic acid alone.
摘要
从成年猪喉部分离出的软骨细胞在使用前作为细胞悬液培养至少4天。在用35SO42-和[3H]葡糖胺作为前体的营养培养基中进行18小时的连续标记实验期间，合成了一些大分子聚阴离子物质，其在凝胶色谱上表现为蛋白聚糖。在琼脂糖2B上进行的凝胶色谱显示，培养基中的一部分蛋白聚糖似乎是聚集的，并在4M-胍盐酸盐中解离。此外，解离的蛋白聚糖与透明质酸相互作用。新合成的蛋白聚糖比单独以糖醛酸测定的平均总十六烷基吡啶氯化物沉淀物质大。
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