Toole B P, Jackson G, Gross J
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1384-6. doi: 10.1073/pnas.69.6.1384.
Purified hyaluronate, at a concentration as low as 1 ng/ml, blocks the formation of colonies and cartilage nodules in stationary cultures of cells, isolated by treatment with trypsin, from embryonic chick somites and limb buds. This phenomenon in vitro is correlated with sequences of hyaluronate production and hyaluronidase activity during chondrogenesis in embryonic and regenerating tissues in vivo. An hypothesis is proposed in which hyaluronate acts as a regulator or inhibitor of mesenchymal cell aggregation in embryogenesis, its synthesis and removal being part of the mechanism of timing of migration, aggregation, and subsequent differentiation.
纯化的透明质酸盐，浓度低至1纳克/毫升时，就能抑制经胰蛋白酶处理从胚胎鸡体节和肢芽分离出的细胞在静止培养物中形成集落和软骨结节。体外的这种现象与体内胚胎和再生组织软骨形成过程中透明质酸盐产生序列及透明质酸酶活性相关。本文提出一个假说，即透明质酸盐在胚胎发育过程中作为间充质细胞聚集的调节剂或抑制剂，其合成与清除是迁移、聚集及后续分化时间调控机制的一部分。
