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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Braidman I P, Gregoriadis G

Biochem J. 1977 May 15;164(2):439-45. doi: 10.1042/bj1640439.

A glucocerebroside beta-glucosidase-rich detergent-free preparation was obtained from human placentas by a rapid method combining affinity chromatography on concanavalin A-Sepharose and organic-solvent precipitation. In a typical preparation about 11000 units of the enzyme purified 1500-fold were obtained from five placentas in 2 days. 2. The enzyme preparation also contained other hydrolases, but the extent of their purification was much smaller. 3. Studies on entrapment in liposomes showed that all glucocerebroside beta-glucosidase activity used could be incorporated in neutral egg phosphatidylcholine-cholesterol liposomes. Association with liposomes appeared to discriminate against other proteins, including some of the hydrolases, thus contributing to further purification of the enzyme. More than 95% of the liposome-associated enzyme activity was latent.

通过将伴刀豆球蛋白A-琼脂糖亲和层析与有机溶剂沉淀相结合的快速方法，从人胎盘中获得了富含葡萄糖脑苷脂β-葡萄糖苷酶的无去污剂制剂。在典型的制备过程中，2天内从5个胎盘中获得了约11000单位、纯化了1500倍的该酶。2. 该酶制剂还含有其他水解酶，但其纯化程度要小得多。3. 对脂质体包封的研究表明，所有使用的葡萄糖脑苷脂β-葡萄糖苷酶活性都可以掺入中性鸡蛋磷脂酰胆碱-胆固醇脂质体中。与脂质体的结合似乎对其他蛋白质具有选择性，包括一些水解酶，从而有助于该酶的进一步纯化。超过95%的脂质体相关酶活性是潜伏性的。

Braidman I P, Gregoriadis G

Biochem J. 1977 May 15;164(2):439-45. doi: 10.1042/bj1640439.

A glucocerebroside beta-glucosidase-rich detergent-free preparation was obtained from human placentas by a rapid method combining affinity chromatography on concanavalin A-Sepharose and organic-solvent precipitation. In a typical preparation about 11000 units of the enzyme purified 1500-fold were obtained from five placentas in 2 days. 2. The enzyme preparation also contained other hydrolases, but the extent of their purification was much smaller. 3. Studies on entrapment in liposomes showed that all glucocerebroside beta-glucosidase activity used could be incorporated in neutral egg phosphatidylcholine-cholesterol liposomes. Association with liposomes appeared to discriminate against other proteins, including some of the hydrolases, thus contributing to further purification of the enzyme. More than 95% of the liposome-associated enzyme activity was latent.

通过将伴刀豆球蛋白A-琼脂糖亲和层析与有机溶剂沉淀相结合的快速方法，从人胎盘中获得了富含葡萄糖脑苷脂β-葡萄糖苷酶的无去污剂制剂。在典型的制备过程中，2天内从5个胎盘中获得了约11000单位、纯化了1500倍的该酶。2. 该酶制剂还含有其他水解酶，但其纯化程度要小得多。3. 对脂质体包封的研究表明，所有使用的葡萄糖脑苷脂β-葡萄糖苷酶活性都可以掺入中性鸡蛋磷脂酰胆碱-胆固醇脂质体中。与脂质体的结合似乎对其他蛋白质具有选择性，包括一些水解酶，从而有助于该酶的进一步纯化。超过95%的脂质体相关酶活性是潜伏性的。