Inoue M, Okajima K, Ito K, Utsumi K, Seno S
Biochim Biophys Acta. 1977 Jun 2;467(2):130-6. doi: 10.1016/0005-2736(77)90190-0.
The effect of concanavalin A and its succinylated derivative on cell agglutination and potassium compartmentation of mature and immature erythrocytes was observed. The binding of tetravalent concanavalin A to the surface glycoproteins of rabbit erythrocytes leads to a change in the properties of the surface membrane, which results in an induction of cell agglutination and concomitant release of potassium from the cells. Both of the phenomena induced by concanavalin A are temperature dependent, and observed at above 15 degrees C. Divalent succinylated concanavalin A, lacking the inducing activity of surface glycoprotein cross-linking into patches and caps, caused neither cell agglutination nor change in the potassium compartmentation of erythrocytes and reticulocytes. In the case of immature reticulocytes, however, remarkable agglutination of the cells was induced without a change in the potassium compartmentation after treatment with tetravalent concanavalin A. It is suggested that changes in the molecular organization of the surface membrane occur in which potassium compartmentation of the reticulocytes becomes more susceptible to surface glycoprotein cross-linking during cellular maturation.
观察了伴刀豆球蛋白A及其琥珀酰化衍生物对成熟和未成熟红细胞的细胞凝集及钾离子分布的影响。四价伴刀豆球蛋白A与兔红细胞表面糖蛋白的结合导致表面膜性质发生变化，进而引发细胞凝集并伴随细胞内钾离子的释放。伴刀豆球蛋白A诱导的这两种现象均与温度有关，在15℃以上可观察到。二价琥珀酰化伴刀豆球蛋白A缺乏将表面糖蛋白交联成斑和帽的诱导活性，既不引起红细胞和网织红细胞的细胞凝集，也不改变其钾离子分布。然而，在用四价伴刀豆球蛋白A处理未成熟网织红细胞后，可诱导细胞发生显著凝集，而钾离子分布无变化。这表明表面膜的分子组织发生了变化，在细胞成熟过程中网织红细胞的钾离子分布变得更容易受到表面糖蛋白交联的影响。
