Mínguez F, Lima J E, García M T, Prieto J
Department of Microbiology, Faculty of Medicine, Complutense University, Madrid, Spain.
Chemotherapy. 1996 Jul-Aug;42(4):273-9. doi: 10.1159/000239455.
This study evaluates the influence of both fresh and heated human serum on the postantifungal effect (PAFE) induced by different concentrations of amphotericin B (AmB), 5-fluorocytosine (5-Fc), Ketoconazole (Kz) and fluconazole (Flu) on two strains of Candida albicans. The concentrations were selected in harmony with the pharmacokinetic properties and toxicity of the drugs. Without serum there was no delay in the growth of yeast cultures pretreated with Kz or Flu, leading to negative PAFEs, however with AmB and 5-Fc the PAFEs were positive. When assays were made in the presence of 10% fresh human serum, the duration of the PAFEs increased with all drugs tested, and those induced by azolic agents became positive. In the presence of 10% human serum heated at 56 degrees C for 30 min, the PAFEs of the antifungal agents were similar to those obtained in the absence of serum. Our results suggest that fresh serum positively influenced PAFE which may be an important factor in determining the dosing regimen for infection by yeasts.
本研究评估了新鲜和加热的人血清对不同浓度的两性霉素B（AmB）、5-氟胞嘧啶（5-Fc）、酮康唑（Kz）和氟康唑（Flu）诱导的抗真菌后效应（PAFE）的影响，受试菌株为两株白色念珠菌。药物浓度的选择与药物的药代动力学特性和毒性相匹配。在无血清的情况下，用Kz或Flu预处理的酵母培养物生长没有延迟，导致PAFE为阴性，然而用AmB和5-Fc处理时PAFE为阳性。当在10%新鲜人血清存在的情况下进行试验时，所有受试药物的PAFE持续时间均增加，并且唑类药物诱导的PAFE变为阳性。在56℃加热30分钟的10%人血清存在的情况下，抗真菌药物的PAFE与无血清时获得的结果相似。我们的结果表明，新鲜血清对PAFE有正向影响，这可能是确定酵母感染给药方案的一个重要因素。
