Mínguez Mínguez F, Lima J E, García M T, Prieto J
Department of Microbiology, School of Medicine, Universidad Complutense, Madrid, Spain.
Chemotherapy. 1997 Sep-Oct;43(5):346-51. doi: 10.1159/000239588.
The aim of this work was to measure the susceptibility of Candida albicans pretreated for 2 or 6 h with fluconazole (Flu), ketoconazole (Ktz), amphotericin B (AmB) and 5-fluorocytosine (5-Fc) to the fungicidal action of human polymorphic leukocytes. The influence of pretreatment was measured by comparing the delay (in hours) and reduction (log10) in growth of pretreated cultures in the presence of leukocytes and serum with that of non-pretreated control cultures. Six-hour pretreatments with Flu, Ktz, AmB and 5-Fc led to growth delays of 1, 3, 3 and 3 h, respectively. No significant differences were found when pretreatment lasted only 2 h. With respect to a reduction in growth, this was larger when the preincubation was 6 h, mainly for 5-Fc and AmB. 5-Fc was seen to be the most effective, followed by Ktz, AmB and finally Flu. It may be concluded that antifungal pretreatment renders this yeast more susceptible to the action of leukocytes. The degree of susceptibility achieved is dependent on the antifungal agent employed.
本研究旨在测定白色念珠菌经氟康唑（Flu）、酮康唑（Ktz）、两性霉素B（AmB）和5-氟胞嘧啶（5-Fc）预处理2小时或6小时后对人多形核白细胞杀菌作用的敏感性。通过比较预处理培养物在白细胞和血清存在下的生长延迟（以小时计）和生长减少（log10）与未预处理对照培养物的情况来测定预处理的影响。用Flu、Ktz、AmB和5-Fc进行6小时预处理分别导致生长延迟1小时、3小时、3小时和3小时。当预处理仅持续2小时时，未发现显著差异。关于生长减少，预孵育6小时时更大些，主要是对于5-Fc和AmB。可见5-Fc最有效，其次是Ktz、AmB，最后是Flu。可以得出结论，抗真菌预处理使这种酵母对白细胞的作用更敏感。所达到的敏感程度取决于所使用的抗真菌剂
