Bloomfield V A
Department of Biochemistry, University of Minnesota, St. Paul 55108, USA.
Curr Opin Struct Biol. 1996 Jun;6(3):334-41. doi: 10.1016/s0959-440x(96)80052-2.
Recent progress in our understanding of DNA condensation includes the observation of the collapse of single DNA molecules, greater insights into the intermolecular forces driving condensation, the recognition of helix-structure perturbation in condensed DNA, and the increasing recognition of the likely biological consequences of condensation. DNA condensed with cationic liposomes is an efficient agent for the transfection of eukaryotic cells, with considerable potential interest for gene therapy.
我们对DNA凝聚的理解取得了新进展,包括观察到单个DNA分子的塌缩、对驱动凝聚的分子间作用力有了更深入的认识、认识到凝聚态DNA中的螺旋结构扰动,以及越来越认识到凝聚可能产生的生物学后果。与阳离子脂质体凝聚的DNA是一种高效的真核细胞转染剂,在基因治疗方面具有相当大的潜在应用价值。